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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated: 2017 Aug. 1
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Warning
– Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite
Conservation Score
Human Protein:
NOS1
All Species:
22.42
Human Site:
T1410
Identified Species:
49.33
UniProt:
P29475
Number Species:
10
Phosphosite Substitution
Charge Score:
0.1
Phosphosite
Sequences
Species
Species
Scientific Name
UniProt ID
NCBI Ref Seq ID
AA#
Mr(Da)
P-Site
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Human
Homo sapiens
P29475
NP_000611.1
1434
160970
T1410
T
L
R
T
Y
E
V
T
N
R
L
R
S
E
S
Chimpanzee
Pan troglodytes
XP_001148238
1153
131137
A1130
Y
H
E
D
I
F
G
A
V
F
P
Y
E
A
K
Rhesus Macaque
Macaca mulatta
XP_001083352
1431
160593
E1398
R
D
D
N
R
Y
H
E
D
I
F
G
V
T
L
Dog
Lupus familis
XP_534695
1431
160627
T1407
T
L
R
T
Y
E
V
T
N
R
L
R
S
E
S
Cat
Felis silvestris
Mouse
Mus musculus
Q9Z0J4
1429
160454
T1405
T
L
R
T
Y
E
V
T
N
R
L
R
S
E
S
Rat
Rattus norvegicus
P29476
1429
160541
T1405
T
L
R
T
Y
E
V
T
N
R
L
R
S
E
S
Wallaby
Macropus eugenll
Platypus
Ornith. anatinus
XP_001506887
1152
131150
R1129
F
Q
L
K
D
Q
R
R
Y
H
E
D
I
F
G
Chicken
Gallus gallus
Q90703
1136
129631
Q1113
Q
A
E
E
Y
F
F
Q
L
K
S
Q
K
R
Y
Frog
Xenopus laevis
NP_001079155
1419
160392
T1395
T
L
R
T
Y
E
V
T
N
R
L
R
S
E
S
Zebra Danio
Brachydanio rerio
NP_571735
1431
161840
T1407
T
L
R
T
C
E
V
T
N
R
L
R
S
E
S
Tiger Blowfish
Takifugu rubipres
Fruit Fly
Dros. melanogaster
Q27571
1349
151696
D1322
D
E
S
R
Y
H
E
D
I
F
G
I
T
L
R
Honey Bee
Apis mellifera
Nematode Worm
Caenorhab. elegans
Sea Urchin
Strong. purpuratus
Poplar Tree
Populus trichocarpa
Maize
Zea mays
Rice
Oryza sativa
Thale Cress
Arabidopsis thaliana
Baker's Yeast
Sacchar. cerevisiae
Red Bread Mold
Neurospora crassa
Conservation
Percent
Protein Identity:
100
45.3
96.1
94.2
N.A.
93.7
93.5
N.A.
44.6
44.7
78
74.3
N.A.
43.4
N.A.
N.A.
N.A.
Protein Similarity:
100
58.5
97.2
96.5
N.A.
95.9
96.3
N.A.
58.8
58.7
88.5
85.3
N.A.
59.2
N.A.
N.A.
N.A.
P-Site Identity:
100
0
0
100
N.A.
100
100
N.A.
0
6.6
100
93.3
N.A.
6.6
N.A.
N.A.
N.A.
P-Site Similarity:
100
0
6.6
100
N.A.
100
100
N.A.
6.6
20
100
93.3
N.A.
13.3
N.A.
N.A.
N.A.
Percent
Protein Identity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
Protein Similarity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
P-Site Identity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
P-Site Similarity:
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
Phosphosite
Consensus
Position
-7
-6
-5
-4
-3
-4
-5
0
+1
+2
+3
+4
+5
+6
+7
% Ala:
0
10
0
0
0
0
0
10
0
0
0
0
0
10
0
% A
% Cys:
0
0
0
0
10
0
0
0
0
0
0
0
0
0
0
% C
% Asp:
10
10
10
10
10
0
0
10
10
0
0
10
0
0
0
% D
% Glu:
0
10
19
10
0
55
10
10
0
0
10
0
10
55
0
% E
% Phe:
10
0
0
0
0
19
10
0
0
19
10
0
0
10
0
% F
% Gly:
0
0
0
0
0
0
10
0
0
0
10
10
0
0
10
% G
% His:
0
10
0
0
0
10
10
0
0
10
0
0
0
0
0
% H
% Ile:
0
0
0
0
10
0
0
0
10
10
0
10
10
0
0
% I
% Lys:
0
0
0
10
0
0
0
0
0
10
0
0
10
0
10
% K
% Leu:
0
55
10
0
0
0
0
0
10
0
55
0
0
10
10
% L
% Met:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% M
% Asn:
0
0
0
10
0
0
0
0
55
0
0
0
0
0
0
% N
% Pro:
0
0
0
0
0
0
0
0
0
0
10
0
0
0
0
% P
% Gln:
10
10
0
0
0
10
0
10
0
0
0
10
0
0
0
% Q
% Arg:
10
0
55
10
10
0
10
10
0
55
0
55
0
10
10
% R
% Ser:
0
0
10
0
0
0
0
0
0
0
10
0
55
0
55
% S
% Thr:
55
0
0
55
0
0
0
55
0
0
0
0
10
10
0
% T
% Val:
0
0
0
0
0
0
55
0
10
0
0
0
10
0
0
% V
% Trp:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% W
% Tyr:
10
0
0
0
64
10
0
0
10
0
0
10
0
0
10
% Y
% Spaces:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% _